s hominis reference strain atcc  (ATCC)

Journal: Nature Communications

Article Title: An antimicrobial daptide from human skin commensal Staphylococcus hominis protects against skin pathogens

doi: 10.1038/s41467-025-66259-w

Figure Lengend Snippet: a, d, f S. aureus strain AH6350 ( a ), S. hominis strains AH4553 ( d ), and AH5011 ( f ) were incubated with 40% CM from AH5011 or TSB for 8 h and monitored by cell density (OD 600 ) and colony-forming units (CFUs). Data from n = 3 biological replicates are reported as the mean and standard deviation. Statistical significance in comparison to the TSB control group was determined using repeated measures two-way analysis of variance (ANOVA). Exact p values from left to right are as follows: a (CFU) 0.0320, 0.0488, a (OD 600 ) < 0.0001, <0.0001, d (CFU) 0.0201, 0.0007, d (OD 600 ) 0.0092, <0.0001, f (CFU) 0.3912, >0.9999, f (OD 600 ) > 0.9999, >0.9999. b Antimicrobial susceptibility of Staphylococcus sp., Micrococcus luteus , Streptococcus pyogenes, Streptococcus agalactiae , and Enterococcus faecalis . “N” values indicate the number of bacterial strains tested which are listed in Supplementary Data . The data represents the mean of n = 2 biological replicates for each strain tested. c Representative images of spot-on-lawn bioactivity of AH5011. Scale bar = 10 mm. e , g S. hominis strains AH4553 ( e ) and AH5011 ( g ) were incubated in 40%, 20%, 10% CM from AH5011 or TSB for 8 h. Data from n = 3 biological replicates are reported as the mean and standard deviation. Statistical differences were compared to TSB control group at 8 h using ordinary one-way ANOVA followed by Dunnett’s multiple comparison test. Exact p values from top to bottom are as follows: e < 0.0001, <0.0001, <0.0001, g 0.9345, 0.8103, 0.7501. Source data are provided as a Source Data file.

Article Snippet: We observed that the antimicrobial function inhibits the S. hominis reference strain ATCC 27844 (AH4553) from the American Type Culture Collection in a concentration-dependent manner (Fig. ).

Techniques: Incubation, Standard Deviation, Comparison, Control

Journal: Nature Communications

Article Title: An antimicrobial daptide from human skin commensal Staphylococcus hominis protects against skin pathogens

doi: 10.1038/s41467-025-66259-w

Figure Lengend Snippet: a Gene organization of the minimal hom BGC (no flanking genes included), with the constructed gene omissions depicted. b Representative images of spot-on-lawn bioactivity of S. aureus RN4220 wild-type (wt) and expression mutants on a bacterial lawn of AH4553, with inhibition zone diameters indicated by black dashed lines. Red dashed line indicates the limit of detection. Scale bar = 10 mm. Data represents the mean of n = 5 biological replicates and error bars show standard deviation. c S. hominis AH4553 was cultured in 40% v/v CM from engineered S. aureus strains for 8 h. Data represents the mean of n = 3 biological replicates and error bars show standard deviation. Statistical significance in comparison to the S. aureus strain expressing empty vector was determined by ordinary one-way ANOVA followed by Dunnett’s multiple comparison test. Exact p values from left to right are as follows: b < 0.0001, <0.0001, 0.9591, 0.9990, >0.9999, 0.3197, c 0.1457, 0.9983, 0.1486, 0.1599, <0.0001, <0.0001. Source data are provided as a Source Data file.

Article Snippet: We observed that the antimicrobial function inhibits the S. hominis reference strain ATCC 27844 (AH4553) from the American Type Culture Collection in a concentration-dependent manner (Fig. ).

Techniques: Construct, Expressing, Inhibition, Standard Deviation, Cell Culture, Comparison, Plasmid Preparation

Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: The sequences of loop-mediated isothermal amplification (LAMP) primers.

Article Snippet: To determine whether the reactions interfered with each other, the reaction chambers in odd numbers were embedded with primers for femA-SHO gene while the chambers in even numbers remained empty during the preparation of the chip, and the reference strain S. hominis ATCC 27844 with a concentration of 10 4 CFU/μl was used as described above.

Techniques: Amplification

Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: Results of the LOD tests. (A1–E1) Amplification curves of the LOD tests for S. aureus , S. epidermidis , S. haemolyticus , S. hominis , and methicillin-resistant S. aureus , respectively, and (A2–E2) the corresponding melting curve analysis. LOD, limit of detection; dF/dT, derivative of the fluorescence/derivative of the temperature; SAU, Staphylococcus aureus ; SEP, Staphylococcus epidermidis ; SHA, Staphylococcus haemolyticus ; SHO, Staphylococcus hominis ; MRSA, methicillin-resistant Staphylococcus aureus ; NTC, no template control.

Article Snippet: To determine whether the reactions interfered with each other, the reaction chambers in odd numbers were embedded with primers for femA-SHO gene while the chambers in even numbers remained empty during the preparation of the chip, and the reference strain S. hominis ATCC 27844 with a concentration of 10 4 CFU/μl was used as described above.

Techniques: Amplification, Fluorescence, Control

Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: Results of the specificity tests. (A1–E1) Amplification curves of the specificity tests for S. aureus , S. epidermidis , S. haemolyticus , S. hominis , and methicillin-resistant S. aureus , respectively, and (A2–E2) the corresponding melting curve analysis. dF/dT, derivative of the fluorescence/derivative of the temperature; SAU, Staphylococcus aureus ; MSSA, methicillin-susceptible Staphylococcus aureus ; SEP, Staphylococcus epidermidis ; SHA, Staphylococcus haemolyticus ; SHO, Staphylococcus hominis ; SWA, Staphylococcus warneri ; SCA, Staphylococcus capitis ; SCO, Staphylococcus cohnii ; MRSA, methicillin-resistant Staphylococcus aureus ; SAR, Staphylococcus argenteus ; SLU, Staphylococcus lugdunensis ; SAP, Staphylococcus saprophyticus ; SAI, Staphylococcus auricularis ; SCH, Staphylococcus chromogenes ; SPE, Staphylococcus pettenkoferi ; SPS, Staphylococcus pseudintermedius ; ECO, Escherichia coli ; KPN, Klebsiella pneumoniae ; PAE, Pseudomonas aeruginosa ; ABA, Acinetobacter baumannii ; EFA, Enterococcus faecium ; ECL, Enterobacter cloacae ; SMA, Stenotrophomonas maltophilia ; SPN, Streptococcus pneumoniae ; ACA, Acinetobacter calcoaceticus ; PMI, Proteus mirabilis ; SPY, Streptococcus pyogenes ; NTC, no template control.

Article Snippet: To determine whether the reactions interfered with each other, the reaction chambers in odd numbers were embedded with primers for femA-SHO gene while the chambers in even numbers remained empty during the preparation of the chip, and the reference strain S. hominis ATCC 27844 with a concentration of 10 4 CFU/μl was used as described above.

Techniques: Amplification, Fluorescence, Control